Proteomics and Signal Transduction / Matthias Mann

The Group

Director
Prof. Dr. Matthias Mann

MaxQuant

Cox J, Mann M., Nat. Biotech. 26, 1367 - 1372.
MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification. more...

Cox J et al., Nat Protoc. 2009;4(5):698-705.
A practical guide to the MaxQuant computational platform for SILAC-based
quantitative proteomics. more...

Precision Mapping of an In Vivo N-Glycoproteome Reveals Rigid Topological and Sequence Constraints.

Cell, Volume 141, Issue 5, 897-907, 28 May 2010
D. Zielinska, F. Gnad, J. Wisniewski, M. Mann - www.cell.com -

[abstract] [phosida]

Decoding signalling networks by mass spectrometry-based proteomics.

Nature Reviews Molecular Cell Biology 11, 427-439 (June 2010)
Chunaram Choudhary and Matthias Mann - www.nature.com -

[abstract]

Super-SILAC mix for quantitative proteomics of human tumor tissue.

Nat Methods. 2010 Apr 4.

Geiger T, Cox J, Ostasiewicz P, Wisniewski JR, Mann M.
- www.nature.com -

[abstract] [Recommended by Faculty of 1000 Biology]

Quantitative Proteomics Reveals Subset-Specific Viral Recognition in Dendritic Cells.

Immunity, Volume 32, Issue 2, 279-289, 18 February 2010

Luber CA, Cox J, Lauterbach H, Fancke B, Selbach M, Tschopp J, Akira S, Wiegand M, Hochrein H, O'Keeffe M, Mann M. - www.cell.com -

[abstract]

Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

Sci. Signal., 12 January 2010
Vol. 3, Issue 104, p. ra3

Olsen JV, Vermeulen M, Santamaria A, Kumar C, Miller ML, Jensen LJ, Gnad F, Cox J, Jensen TS, Nigg EA, Brunak S, Mann M. - www.sciencemag.org -

[abstract]

Quantitative proteomic analysis of single pancreatic islets.

Waanders LF, Chwalek K, Monetti M, Kumar C, Lammert E, Mann M.,
Proc Natl Acad Sci U S A. 2009 Oct 21. - www.pnas.org

[abstract]

Lysine acetylation targets protein complexes and co-regulates major cellular functions.

Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.,Science 14 August 2009: 834-840. - www.sciencemag.org -

[abstract]

BREAKTHROUGH OF THE YEAR:The Runners-Up

Science news stories - www.sciencemag.org -
Sequencing and quantitation of the first entire yeast proteome was selected by Science as one of the 10 Breakthroughs of the year. (See the paper here and see item 5 in "Watching proteins at work"). more...

Comprehensive mass-spectrometry-based proteome quantification of haploid versus diploid yeast

L. M. F. de Godoy et al., Nature 455, 1251-1254 (2008)

Mass spectrometry is a powerful technology for the analysis of large numbers of endogenous proteins. However, the analytical challenges associated with comprehensive identification and relative quantification of cellular proteomes have so far appeared to be insurmountable. Here, using advances in computational proteomics, instrument performance and sample preparation strategies, we compare protein levels of essentially all endogenous proteins in haploid yeast cells to their diploid counterparts. more...

MaxQuant Summerschool 2010

the FASP procedure

Wisniewski J.R. et al.
Nat. Methods 6:359-362.

Filter Aided Sample Preparation (FASP) method allows proteomic analysis of SDS-lysates with essentially any class of proteins and origin of biological material. In particular, FASP enables digestion of membrane proteins under conditions previously applied only to soluble proteins.
more...