How the screen was done
In short, we treated Drosophila S2 cells with interfereing RNAi's to knock down expression of a single gene at a time. After 3 days, we induced the formation of lipid droplets by adding oleate containing medium (see also movie). After 13 h cells were fixed, stained for lipid droplets with BODIPY and for nuclei with DAPI, imaged in a high throughput microscope and scored visually and with an autmated image analysis algorithm. Potential hits were treated with a second set of siRNA's and processed the same way.....
Hits that were confirmed with an independent set of siRNA's were sorted into 5 phenotypic classes as indicated below. Images form the screen sorted according to the class they belong to can be found at the "Galleries of hits" page.
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